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Financement de l’UE (2 988 514 €) : Technologies Raman ultrarapides pour l’identification et le séquençage des protéines Hor28/01/2021 Programme de recherche et d'innovation de l'UE « Horizon »
Texte
Technologies Raman ultrarapides pour l’identification et le séquençage des protéines
The human proteome is the whole set of protein that a human can potentially express. Most of the human proteome is known. However the proteome, being the set of proteins potentially expressed, does not give information regarding the protein really expressed in a specific person or a patient. The possibility of accessing to this fundamental information through a cost and time effective technique will revolutionize our ability to prevent, diagnose and treat most of the human diseases. This is the commitment of ProID that aims to provide a technological platform able to record single protein Raman spectra with single amino-acid resolution. Namely, by reading the sequence of selected amino-acids along the protein chain, the platform will identify the corresponding protein. To reach this goal we want to combine advanced nanofabrications of plasmonic nanopores with ultrafast time resolved photon detectors and machine learning algorithms. More in details: i) plasmonic nanopores will be exploited to achieve single amino-acid optical excitation and enhanced Raman stimulation; ii) ultrafast and ultrasensitive Raman spectrometers will be obtained by combining the emerging technologies of SPAD (Single Photon Avalanche Diode) arrays with dedicated otpica elements which improve the sensitivity and the speed of the detector, and reduce the number of elements of the array necessary to sample a Raman spectrum; iii) bioinformatics approaches will complete the technological platform by developing specific software for discriminating the Raman spectra of proteins with reduced spectral points. Also, insightful experiments on electrophoretic translocation and augmented fluid viscosity in ultra-confined systems will contribute the molecular motion into the nanopores. Finally, to state of the art in-silico design will support the project by contributing to system optimization and data analyses/interpretation.
| Centre National de la Recherche Scientifique CNRS | 225 000 € |
| EUROPEAN MOLECULAR BIOLOGY LABORATORY | 290 950 € |
| Fondazione Istituto Italiano Di Tecnologia | 916 031 € |
| KARLSRUHER INSTITUT FUER TECHNOLOGIE | 306 250 € |
| Micro Photon Devices Srl | 25 000 € |
| Politecnico Di Milano | 376 460 € |
| Umea Universitet | 0,00 € |
| Universita Degli Studi Di Padova | 250 125 € |
| Universite Du Luxembourg | 598 698 € |
https://cordis.europa.eu/project/id/964363
Cette annonce se réfère à une date antérieure et ne reflète pas nécessairement l’état actuel.