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Financement britannique (83 418 £) : CCC non synchrone Ukri 01/12/2006 Recherche et innovation du Royaume-Uni, Royaume Uni
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CCC non synchrone
| Abstract | There is a current increase in the research emphasis of pharmaceutical companies into the rapid, cost effective manufacture of a new generation of biopharmaceutical therapies and in particular to facilitate 'downstream processing with fewer process steps that preserves macromolecular structure and integrity.' (DTI BIGT report: 'Bioscience 2015'). Dynamic Extraction (DE) is a liquid-liquid separation technique that, unlike traditional chromatography, does not need a solid support. Instead, it uses two immiscible (usually aqueous/organic) liquids; one liquid is retained in the coiled column by centrifugal force, while the other is continuously pumped past it. Rotation of the coiled tubes relative to the rotor itself causes the mixing and settling of the two immiscible liquids that results in the partition/distribution process. If the equipment could be improved to enable the separation of biopharmaceuticals using non-organic solvents, DE could become a 'green', low-cost alternative for conventional high-resolution separations both at laboratory and industrial scale. In principle, the column can be rotated at any speed relative to the rotor. However a further feature that makes DE centrifuges ideal for bio-separations is the 'flying-lead', an anti-twist mechanism that allows external equipment to be connected to the spinning column by a continuous flexible tube thus avoiding problems of over-heating of sensitive biological molecules and contamination. This is achieved by spinning the coiled column in a synchronous planetary motion generated by a 1:1 simple planet-gear drive. Attempts to move towards the use of 'green' solvents in DE have met with some problems; the small density difference between, and high viscosity of, suitable 'bio-friendly' liquids means that a large centrifugal force is required both to retain them in the tube and to separate them. Unfortunately, the current 1:1 flying-lead mechanism requires that the coiled tube rotates at the same speed as the rotor and the resulting rapid mixing and settling may both damage bio-molecules and emulsify the solvent liquids instead of keeping them as two separate layers. The aim of this project is to overcome these problems and establish the basis for extending the capabilities of the DE process to enable the efficient recovery and purification of next generation of bio-therapeutics. It will involve the design and construction of a new generation of DE device that incorporates non-synchronous rotation between the main rotor and the coiled tube (i.e. where the mixing speed is decoupled from the centrifugal force) but which retains all the other intrinsic advantages of the technology, such as the flying lead connection. We have devised a novel way of making such a non-synchronous centrifuge by incorporating a second anti-twist mechanism between the gear for the centrifuge rotor and the coiled tube, allowing them to spin at different speeds. This arrangement, and the simple manner in which we achieve the additional anti-twist, are the basis for the novelty of our proposed scheme. |
| Category | Research Grant |
| Reference | BB/E012949/1 |
| Status | Closed |
| Funded period start | 01/12/2006 |
| Funded period end | 31/07/2008 |
| Funded value | £83 418,00 |
| Source | https://gtr.ukri.org/projects?ref=BB%2FE012949%2F1 |
Participating Organisations
| BRUNEL UNIVERSITY LONDON | |
| Dynamic Extractions |
Cette annonce se réfère à une date antérieure et ne reflète pas nécessairement l’état actuel. L’état actuel est présenté à la page suivante : Brunel University London, Uxbridge, Royaume Uni.