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Financement de l’UE (3 151 750 €) : Un MicroNanoBioSystem et un instrument portables pour l’analyse ultra-rapide des agents pathogènes dans les aliments : l’innovation du prototype de laboratoire … Hor01/02/2016 Programme de recherche et d'innovation de l'UE « Horizon »
Texte
Un MicroNanoBioSystem et un instrument portables pour l’analyse ultra-rapide des agents pathogènes dans les aliments : l’innovation du prototype de laboratoire LOVE-FOOD à un instrument pré-commercial
The development of new methodologies advancing the state of the art in foodborne pathogen detection is a challenge for scientists and technologists as well as food industry and consumers. This project aims to meet the challenge by providing a reliable and versatile solution thanks to the convergence of micro-nano-bio systems. The work capitalizes on several innovative concepts which have already been proven to meet the required criteria for fast, low cost and highly sensitive analysis of pathogens in food samples in a previous research project entitled LoveFood. These concepts are gathered on a credit-card size Lab-on-Chip platform, where all necessary steps for bacteria detection are performed on several chips. Specifically, bacteria capture and lysis (one chip), DNA extraction (second chip) and amplification (third chip) and finally pathogenic-DNA detection (fourth chip) can be performed in less than 8 hours and without the need for skilled personnel or large, lab-based dedicated equipment. To proceed for a higher Technology Readiness Level towards the successful commercialization of the current prototype and produce a portable, and rapid platform (targeting total pathogen analysis time 4 hours including a 3 hour pre-enrichment step), we propose to further develop it by integrating the bacteria lysis, DNA purification and amplification modules, as well as the biochip detection platform on a single cartridge, able to perform multi-pathogen analysis (i.e. Salmonella, Listeria, Escherichia coli and Bacillus cereus) in multiple samples. The system will be developed for dairy products and meat analysis, with a strong commitment to produce a pre-industrial prototype by the end of the project.
| Idryma Technologias Kai Erevnas | 809 791 € |
| Institut Pasteur | 340 000 € |
| JOBST TECHNOLOGIES GmbH | 316 750 € |
| Makedoniki Viomihania Galaktos Anonimos Eteria (Mevgal SA) | 42 000 € |
| National Center FOR Scientific Research Demokritos | 994 834 € |
| Senseor SAS | 394 875 € |
| Univerzita Pardubice | 253 500 € |
https://cordis.europa.eu/project/id/687681
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