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Financement de l’UE (3 730 000 €) : Spectrométrie de masse pour l’imagerie de particules uniques de complexes protéiques orientés dipolaires Hor28/06/2018 Programme de recherche et d'innovation de l'UE « Horizon »
Vue d’ensemble
Texte
Spectrométrie de masse pour l’imagerie de particules uniques de complexes protéiques orientés dipolaires
The European XFEL has just entered user operation. With its unparalleled peak brilliance and repetition rate, European XFEL has the potential to further applications in single particle imaging (SPI), thus far limited to large viral particles at X-ray Free-Electron Lasers (XFEL). SPI will allow imaging protein complexes without the need for crystallization. This eventually renders transient conformational states accessible for high resolution structural studies yielding molecular movies of biomolecular machines. A major bottleneck is the wealth of data required to reconstruct a single structure leading to long processing times. This is currently also a problem in electron microscopy (EM). MS SPIDOC will overcome this data challenge by developing a native mass spectrometry (MS) system for sample delivery, named X-MS-I. It will provide mass and conformation selected biomolecules, which are oriented along their dipole axis upon imaging. This will enable structural reconstruction from much smaller datasets speeding up the analysis time tremendously. Moreover, the system features low sample consumption and a controlled low background easing pattern identification. The main objectives of the project are: • Deliver mass and conformation separated biomolecules for SPI. • Orient proteins for SPI. • Image protein complex unfolding • Exploit potential of protein orientation for other applications The MS SPIDOC consortium combines internationally leading expertise in different fields relevant to the project: Instrument design and development, computer simulations as well as working with biomolecules in the gas phase and on SPI are combined to implement the novel sample environment at the next generation XFEL facility. New components and methods will be opened to the market and thereby strengthen the European Research Area (ERA) and industry. This early stage high-risk project will give rise to a new technology with major impact on how to derive structures of biomolecules.
| Centre National de la Recherche Scientifique CNRS | 0,00 € |
| European X-RAY Free-Electron Laserfacility GmbH | 600 000 € |
| Fasmatech Epistimoniki Kai Technologiki ΑΕ | 587 500 € |
| Leibniz-Institut FUR Virologie | 499 125 € |
| Spectrometry Vision B.V. | 432 500 € |
| The University of Manchester | 362 375 € |
| Universitaet Greifswald | 421 000 € |
| Universite Lyon 1 Claude Bernard | 315 000 € |
| Uppsala Universitet | 512 500 € |
https://cordis.europa.eu/project/id/801406
Cette annonce se réfère à une date antérieure et ne reflète pas nécessairement l’état actuel.
